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pcdna3 1 zeo expression vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcdna3 1 zeo expression vector
    Pcdna3 1 Zeo Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3%2E1+expression+vector/Zeocin/pm41390560-150-188-192
    Average 99 stars, based on 1 article reviews
    pcdna3 1 zeo expression vector - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: The assembly and activation of the PANoptosome promote porcine granulosa cell programmed cell death during follicular atresia
    Article Snippet: The coding sequences of the ZBP1 , RIPK3 , and RIPK1 genes, as well as those of their truncation mutants, were amplified from GC cDNA and cloned into the pcDNA3.1 expression vector (Invitrogen).

    Article Title: Generating and characterizing a comprehensive panel of CHO cells glycosylation mutants for advancing glycobiology and biotechnology research
    Article Snippet: The human erythropoietin (EPO) open reading frame (ORF) was cloned into the pcDNA3.1 expression vector (Thermo Fisher Scientific).

    Article Title: Fusobacterium Nucleatum Promotes Microsatellite Instability in Colorectal Carcinoma Through Up-regulation of miRNA-155-5p-Targeted Inhibition of MSH6 via the TLR4/NF-κB Signaling Pathway.
    Article Snippet: Fusobacterium nucleatum (Fn) is significantly associated with poor prognosis in colorectal carcinoma (CRC), however, mechanisms of Fn in DNA mismatch repair (MMR) and microsatellite instability (MSI) in CRC have not been fully elucidated.. Clinical samples are collected to analyze the relationship between Fn abundance and microsatellite stability.. Tumor cells are treated with Fn to detect the expression of proteins related to toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (Myd88), mutS homolog 6 (MSH6), and nuclear factor-κB (NF-κB) signaling pathways, respectively.

    Article Title: Inhibition of myotube formation by platelet-derived growth factor subunit B in QM7 cells
    Article Snippet: Finally, the amplified sequence was inserted into the multicloning site of the pcDNA3.1 expression vector (Invitrogen, Grand Island, NY, USA).

    Article Title: Long non-coding RNA AC105118.1 affects glycolysis to facilitate oxaliplatin resistance in colorectal cancer cells by modulating the miR-378a-3p/KIF26B axis.
    Article Snippet: Background: Oxaliplatin is a first-line chemotherapy drug for colorectal cancer (CRC), but many patients eventually lose treatment efficacy due to acquired resistance.. AC105118.1 is a long non-coding RNA with unknown biological function.. This research attempts to probe into the molecular regulatory mechanism of AC105118.1 in CRC oxaliplatin resistance.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms.
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN-7, -9, and -12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) (Fig. 4).

    Article Title: LncRNA-NEAT1 facilitates autophagy to boost pemetrexed resistance in lung adenocarcinoma via the mir-379-3p/HIF1A pathway.
    Article Snippet: Background: As a primary chemotherapeutic agent for lung adenocarcinoma (LUAD), pemetrexed (PEM) faces the challenge of resistance development in cancer cells due to its chronic use, which compromises its therapeutic benefits.. LncRNA-NEAT1, implicated in the promotion of cancer, is a key player in LUAD.. The objective of this study is to explore the contribution of lncRNA-NEAT1 to PEM resistance in LUAD and to dissect the molecular mechanisms involved.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN - 7, -9, and - 12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) ( ).

    Sequencing:

    Article Title: The assembly and activation of the PANoptosome promote porcine granulosa cell programmed cell death during follicular atresia
    Article Snippet: The coding sequences of the ZBP1 , RIPK3 , and RIPK1 genes, as well as those of their truncation mutants, were amplified from GC cDNA and cloned into the pcDNA3.1 expression vector (Invitrogen).

    Article Title: Generating and characterizing a comprehensive panel of CHO cells glycosylation mutants for advancing glycobiology and biotechnology research
    Article Snippet: The human erythropoietin (EPO) open reading frame (ORF) was cloned into the pcDNA3.1 expression vector (Thermo Fisher Scientific).

    Article Title: Fusobacterium Nucleatum Promotes Microsatellite Instability in Colorectal Carcinoma Through Up-regulation of miRNA-155-5p-Targeted Inhibition of MSH6 via the TLR4/NF-κB Signaling Pathway.
    Article Snippet: Fusobacterium nucleatum (Fn) is significantly associated with poor prognosis in colorectal carcinoma (CRC), however, mechanisms of Fn in DNA mismatch repair (MMR) and microsatellite instability (MSI) in CRC have not been fully elucidated.. Clinical samples are collected to analyze the relationship between Fn abundance and microsatellite stability.. Tumor cells are treated with Fn to detect the expression of proteins related to toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (Myd88), mutS homolog 6 (MSH6), and nuclear factor-κB (NF-κB) signaling pathways, respectively.

    Article Title: Inhibition of myotube formation by platelet-derived growth factor subunit B in QM7 cells
    Article Snippet: Finally, the amplified sequence was inserted into the multicloning site of the pcDNA3.1 expression vector (Invitrogen, Grand Island, NY, USA).

    Article Title: Long non-coding RNA AC105118.1 affects glycolysis to facilitate oxaliplatin resistance in colorectal cancer cells by modulating the miR-378a-3p/KIF26B axis.
    Article Snippet: Background: Oxaliplatin is a first-line chemotherapy drug for colorectal cancer (CRC), but many patients eventually lose treatment efficacy due to acquired resistance.. AC105118.1 is a long non-coding RNA with unknown biological function.. This research attempts to probe into the molecular regulatory mechanism of AC105118.1 in CRC oxaliplatin resistance.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms.
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN-7, -9, and -12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) (Fig. 4).

    Article Title: LncRNA-NEAT1 facilitates autophagy to boost pemetrexed resistance in lung adenocarcinoma via the mir-379-3p/HIF1A pathway.
    Article Snippet: Background: As a primary chemotherapeutic agent for lung adenocarcinoma (LUAD), pemetrexed (PEM) faces the challenge of resistance development in cancer cells due to its chronic use, which compromises its therapeutic benefits.. LncRNA-NEAT1, implicated in the promotion of cancer, is a key player in LUAD.. The objective of this study is to explore the contribution of lncRNA-NEAT1 to PEM resistance in LUAD and to dissect the molecular mechanisms involved.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN - 7, -9, and - 12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) ( ).

    Expressing:

    Article Title: The assembly and activation of the PANoptosome promote porcine granulosa cell programmed cell death during follicular atresia
    Article Snippet: The coding sequences of the ZBP1 , RIPK3 , and RIPK1 genes, as well as those of their truncation mutants, were amplified from GC cDNA and cloned into the pcDNA3.1 expression vector (Invitrogen).

    Article Title: Generating and characterizing a comprehensive panel of CHO cells glycosylation mutants for advancing glycobiology and biotechnology research
    Article Snippet: The human erythropoietin (EPO) open reading frame (ORF) was cloned into the pcDNA3.1 expression vector (Thermo Fisher Scientific).

    Article Title: Fusobacterium Nucleatum Promotes Microsatellite Instability in Colorectal Carcinoma Through Up-regulation of miRNA-155-5p-Targeted Inhibition of MSH6 via the TLR4/NF-κB Signaling Pathway.
    Article Snippet: Fusobacterium nucleatum (Fn) is significantly associated with poor prognosis in colorectal carcinoma (CRC), however, mechanisms of Fn in DNA mismatch repair (MMR) and microsatellite instability (MSI) in CRC have not been fully elucidated.. Clinical samples are collected to analyze the relationship between Fn abundance and microsatellite stability.. Tumor cells are treated with Fn to detect the expression of proteins related to toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (Myd88), mutS homolog 6 (MSH6), and nuclear factor-κB (NF-κB) signaling pathways, respectively.

    Article Title: Inhibition of myotube formation by platelet-derived growth factor subunit B in QM7 cells
    Article Snippet: Finally, the amplified sequence was inserted into the multicloning site of the pcDNA3.1 expression vector (Invitrogen, Grand Island, NY, USA).

    Article Title: Long non-coding RNA AC105118.1 affects glycolysis to facilitate oxaliplatin resistance in colorectal cancer cells by modulating the miR-378a-3p/KIF26B axis.
    Article Snippet: Background: Oxaliplatin is a first-line chemotherapy drug for colorectal cancer (CRC), but many patients eventually lose treatment efficacy due to acquired resistance.. AC105118.1 is a long non-coding RNA with unknown biological function.. This research attempts to probe into the molecular regulatory mechanism of AC105118.1 in CRC oxaliplatin resistance.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms.
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN-7, -9, and -12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) (Fig. 4).

    Article Title: LncRNA-NEAT1 facilitates autophagy to boost pemetrexed resistance in lung adenocarcinoma via the mir-379-3p/HIF1A pathway.
    Article Snippet: Background: As a primary chemotherapeutic agent for lung adenocarcinoma (LUAD), pemetrexed (PEM) faces the challenge of resistance development in cancer cells due to its chronic use, which compromises its therapeutic benefits.. LncRNA-NEAT1, implicated in the promotion of cancer, is a key player in LUAD.. The objective of this study is to explore the contribution of lncRNA-NEAT1 to PEM resistance in LUAD and to dissect the molecular mechanisms involved.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN - 7, -9, and - 12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) ( ).

    Plasmid Preparation:

    Article Title: The assembly and activation of the PANoptosome promote porcine granulosa cell programmed cell death during follicular atresia
    Article Snippet: The coding sequences of the ZBP1 , RIPK3 , and RIPK1 genes, as well as those of their truncation mutants, were amplified from GC cDNA and cloned into the pcDNA3.1 expression vector (Invitrogen).

    Article Title: Generating and characterizing a comprehensive panel of CHO cells glycosylation mutants for advancing glycobiology and biotechnology research
    Article Snippet: The human erythropoietin (EPO) open reading frame (ORF) was cloned into the pcDNA3.1 expression vector (Thermo Fisher Scientific).

    Article Title: Fusobacterium Nucleatum Promotes Microsatellite Instability in Colorectal Carcinoma Through Up-regulation of miRNA-155-5p-Targeted Inhibition of MSH6 via the TLR4/NF-κB Signaling Pathway.
    Article Snippet: Fusobacterium nucleatum (Fn) is significantly associated with poor prognosis in colorectal carcinoma (CRC), however, mechanisms of Fn in DNA mismatch repair (MMR) and microsatellite instability (MSI) in CRC have not been fully elucidated.. Clinical samples are collected to analyze the relationship between Fn abundance and microsatellite stability.. Tumor cells are treated with Fn to detect the expression of proteins related to toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (Myd88), mutS homolog 6 (MSH6), and nuclear factor-κB (NF-κB) signaling pathways, respectively.

    Article Title: Inhibition of myotube formation by platelet-derived growth factor subunit B in QM7 cells
    Article Snippet: Finally, the amplified sequence was inserted into the multicloning site of the pcDNA3.1 expression vector (Invitrogen, Grand Island, NY, USA).

    Article Title: Long non-coding RNA AC105118.1 affects glycolysis to facilitate oxaliplatin resistance in colorectal cancer cells by modulating the miR-378a-3p/KIF26B axis.
    Article Snippet: Background: Oxaliplatin is a first-line chemotherapy drug for colorectal cancer (CRC), but many patients eventually lose treatment efficacy due to acquired resistance.. AC105118.1 is a long non-coding RNA with unknown biological function.. This research attempts to probe into the molecular regulatory mechanism of AC105118.1 in CRC oxaliplatin resistance.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms.
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN-7, -9, and -12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) (Fig. 4).

    Article Title: LncRNA-NEAT1 facilitates autophagy to boost pemetrexed resistance in lung adenocarcinoma via the mir-379-3p/HIF1A pathway.
    Article Snippet: Background: As a primary chemotherapeutic agent for lung adenocarcinoma (LUAD), pemetrexed (PEM) faces the challenge of resistance development in cancer cells due to its chronic use, which compromises its therapeutic benefits.. LncRNA-NEAT1, implicated in the promotion of cancer, is a key player in LUAD.. The objective of this study is to explore the contribution of lncRNA-NEAT1 to PEM resistance in LUAD and to dissect the molecular mechanisms involved.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN - 7, -9, and - 12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) ( ).

    Synthesized:

    Article Title: The assembly and activation of the PANoptosome promote porcine granulosa cell programmed cell death during follicular atresia
    Article Snippet: The coding sequences of the ZBP1 , RIPK3 , and RIPK1 genes, as well as those of their truncation mutants, were amplified from GC cDNA and cloned into the pcDNA3.1 expression vector (Invitrogen).

    Article Title: Generating and characterizing a comprehensive panel of CHO cells glycosylation mutants for advancing glycobiology and biotechnology research
    Article Snippet: The human erythropoietin (EPO) open reading frame (ORF) was cloned into the pcDNA3.1 expression vector (Thermo Fisher Scientific).

    Article Title: Fusobacterium Nucleatum Promotes Microsatellite Instability in Colorectal Carcinoma Through Up-regulation of miRNA-155-5p-Targeted Inhibition of MSH6 via the TLR4/NF-κB Signaling Pathway.
    Article Snippet: Fusobacterium nucleatum (Fn) is significantly associated with poor prognosis in colorectal carcinoma (CRC), however, mechanisms of Fn in DNA mismatch repair (MMR) and microsatellite instability (MSI) in CRC have not been fully elucidated.. Clinical samples are collected to analyze the relationship between Fn abundance and microsatellite stability.. Tumor cells are treated with Fn to detect the expression of proteins related to toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (Myd88), mutS homolog 6 (MSH6), and nuclear factor-κB (NF-κB) signaling pathways, respectively.

    Article Title: Inhibition of myotube formation by platelet-derived growth factor subunit B in QM7 cells
    Article Snippet: Finally, the amplified sequence was inserted into the multicloning site of the pcDNA3.1 expression vector (Invitrogen, Grand Island, NY, USA).

    Article Title: Long non-coding RNA AC105118.1 affects glycolysis to facilitate oxaliplatin resistance in colorectal cancer cells by modulating the miR-378a-3p/KIF26B axis.
    Article Snippet: Background: Oxaliplatin is a first-line chemotherapy drug for colorectal cancer (CRC), but many patients eventually lose treatment efficacy due to acquired resistance.. AC105118.1 is a long non-coding RNA with unknown biological function.. This research attempts to probe into the molecular regulatory mechanism of AC105118.1 in CRC oxaliplatin resistance.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms.
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN-7, -9, and -12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) (Fig. 4).

    Article Title: LncRNA-NEAT1 facilitates autophagy to boost pemetrexed resistance in lung adenocarcinoma via the mir-379-3p/HIF1A pathway.
    Article Snippet: Background: As a primary chemotherapeutic agent for lung adenocarcinoma (LUAD), pemetrexed (PEM) faces the challenge of resistance development in cancer cells due to its chronic use, which compromises its therapeutic benefits.. LncRNA-NEAT1, implicated in the promotion of cancer, is a key player in LUAD.. The objective of this study is to explore the contribution of lncRNA-NEAT1 to PEM resistance in LUAD and to dissect the molecular mechanisms involved.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN - 7, -9, and - 12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) ( ).

    Clone Assay:

    Article Title: The assembly and activation of the PANoptosome promote porcine granulosa cell programmed cell death during follicular atresia
    Article Snippet: The coding sequences of the ZBP1 , RIPK3 , and RIPK1 genes, as well as those of their truncation mutants, were amplified from GC cDNA and cloned into the pcDNA3.1 expression vector (Invitrogen).

    Article Title: Generating and characterizing a comprehensive panel of CHO cells glycosylation mutants for advancing glycobiology and biotechnology research
    Article Snippet: The human erythropoietin (EPO) open reading frame (ORF) was cloned into the pcDNA3.1 expression vector (Thermo Fisher Scientific).

    Article Title: Fusobacterium Nucleatum Promotes Microsatellite Instability in Colorectal Carcinoma Through Up-regulation of miRNA-155-5p-Targeted Inhibition of MSH6 via the TLR4/NF-κB Signaling Pathway.
    Article Snippet: Fusobacterium nucleatum (Fn) is significantly associated with poor prognosis in colorectal carcinoma (CRC), however, mechanisms of Fn in DNA mismatch repair (MMR) and microsatellite instability (MSI) in CRC have not been fully elucidated.. Clinical samples are collected to analyze the relationship between Fn abundance and microsatellite stability.. Tumor cells are treated with Fn to detect the expression of proteins related to toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (Myd88), mutS homolog 6 (MSH6), and nuclear factor-κB (NF-κB) signaling pathways, respectively.

    Article Title: Inhibition of myotube formation by platelet-derived growth factor subunit B in QM7 cells
    Article Snippet: Finally, the amplified sequence was inserted into the multicloning site of the pcDNA3.1 expression vector (Invitrogen, Grand Island, NY, USA).

    Article Title: Long non-coding RNA AC105118.1 affects glycolysis to facilitate oxaliplatin resistance in colorectal cancer cells by modulating the miR-378a-3p/KIF26B axis.
    Article Snippet: Background: Oxaliplatin is a first-line chemotherapy drug for colorectal cancer (CRC), but many patients eventually lose treatment efficacy due to acquired resistance.. AC105118.1 is a long non-coding RNA with unknown biological function.. This research attempts to probe into the molecular regulatory mechanism of AC105118.1 in CRC oxaliplatin resistance.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms.
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN-7, -9, and -12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) (Fig. 4).

    Article Title: LncRNA-NEAT1 facilitates autophagy to boost pemetrexed resistance in lung adenocarcinoma via the mir-379-3p/HIF1A pathway.
    Article Snippet: Background: As a primary chemotherapeutic agent for lung adenocarcinoma (LUAD), pemetrexed (PEM) faces the challenge of resistance development in cancer cells due to its chronic use, which compromises its therapeutic benefits.. LncRNA-NEAT1, implicated in the promotion of cancer, is a key player in LUAD.. The objective of this study is to explore the contribution of lncRNA-NEAT1 to PEM resistance in LUAD and to dissect the molecular mechanisms involved.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN - 7, -9, and - 12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) ( ).

    Construct:

    Article Title: The assembly and activation of the PANoptosome promote porcine granulosa cell programmed cell death during follicular atresia
    Article Snippet: The coding sequences of the ZBP1 , RIPK3 , and RIPK1 genes, as well as those of their truncation mutants, were amplified from GC cDNA and cloned into the pcDNA3.1 expression vector (Invitrogen).

    Article Title: Generating and characterizing a comprehensive panel of CHO cells glycosylation mutants for advancing glycobiology and biotechnology research
    Article Snippet: The human erythropoietin (EPO) open reading frame (ORF) was cloned into the pcDNA3.1 expression vector (Thermo Fisher Scientific).

    Article Title: Fusobacterium Nucleatum Promotes Microsatellite Instability in Colorectal Carcinoma Through Up-regulation of miRNA-155-5p-Targeted Inhibition of MSH6 via the TLR4/NF-κB Signaling Pathway.
    Article Snippet: Fusobacterium nucleatum (Fn) is significantly associated with poor prognosis in colorectal carcinoma (CRC), however, mechanisms of Fn in DNA mismatch repair (MMR) and microsatellite instability (MSI) in CRC have not been fully elucidated.. Clinical samples are collected to analyze the relationship between Fn abundance and microsatellite stability.. Tumor cells are treated with Fn to detect the expression of proteins related to toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (Myd88), mutS homolog 6 (MSH6), and nuclear factor-κB (NF-κB) signaling pathways, respectively.

    Article Title: Inhibition of myotube formation by platelet-derived growth factor subunit B in QM7 cells
    Article Snippet: Finally, the amplified sequence was inserted into the multicloning site of the pcDNA3.1 expression vector (Invitrogen, Grand Island, NY, USA).

    Article Title: Long non-coding RNA AC105118.1 affects glycolysis to facilitate oxaliplatin resistance in colorectal cancer cells by modulating the miR-378a-3p/KIF26B axis.
    Article Snippet: Background: Oxaliplatin is a first-line chemotherapy drug for colorectal cancer (CRC), but many patients eventually lose treatment efficacy due to acquired resistance.. AC105118.1 is a long non-coding RNA with unknown biological function.. This research attempts to probe into the molecular regulatory mechanism of AC105118.1 in CRC oxaliplatin resistance.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms.
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN-7, -9, and -12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) (Fig. 4).

    Article Title: LncRNA-NEAT1 facilitates autophagy to boost pemetrexed resistance in lung adenocarcinoma via the mir-379-3p/HIF1A pathway.
    Article Snippet: Background: As a primary chemotherapeutic agent for lung adenocarcinoma (LUAD), pemetrexed (PEM) faces the challenge of resistance development in cancer cells due to its chronic use, which compromises its therapeutic benefits.. LncRNA-NEAT1, implicated in the promotion of cancer, is a key player in LUAD.. The objective of this study is to explore the contribution of lncRNA-NEAT1 to PEM resistance in LUAD and to dissect the molecular mechanisms involved.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN - 7, -9, and - 12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) ( ).

    Negative Control:

    Article Title: The assembly and activation of the PANoptosome promote porcine granulosa cell programmed cell death during follicular atresia
    Article Snippet: The coding sequences of the ZBP1 , RIPK3 , and RIPK1 genes, as well as those of their truncation mutants, were amplified from GC cDNA and cloned into the pcDNA3.1 expression vector (Invitrogen).

    Article Title: Generating and characterizing a comprehensive panel of CHO cells glycosylation mutants for advancing glycobiology and biotechnology research
    Article Snippet: The human erythropoietin (EPO) open reading frame (ORF) was cloned into the pcDNA3.1 expression vector (Thermo Fisher Scientific).

    Article Title: Fusobacterium Nucleatum Promotes Microsatellite Instability in Colorectal Carcinoma Through Up-regulation of miRNA-155-5p-Targeted Inhibition of MSH6 via the TLR4/NF-κB Signaling Pathway.
    Article Snippet: Fusobacterium nucleatum (Fn) is significantly associated with poor prognosis in colorectal carcinoma (CRC), however, mechanisms of Fn in DNA mismatch repair (MMR) and microsatellite instability (MSI) in CRC have not been fully elucidated.. Clinical samples are collected to analyze the relationship between Fn abundance and microsatellite stability.. Tumor cells are treated with Fn to detect the expression of proteins related to toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (Myd88), mutS homolog 6 (MSH6), and nuclear factor-κB (NF-κB) signaling pathways, respectively.

    Article Title: Inhibition of myotube formation by platelet-derived growth factor subunit B in QM7 cells
    Article Snippet: Finally, the amplified sequence was inserted into the multicloning site of the pcDNA3.1 expression vector (Invitrogen, Grand Island, NY, USA).

    Article Title: Long non-coding RNA AC105118.1 affects glycolysis to facilitate oxaliplatin resistance in colorectal cancer cells by modulating the miR-378a-3p/KIF26B axis.
    Article Snippet: Background: Oxaliplatin is a first-line chemotherapy drug for colorectal cancer (CRC), but many patients eventually lose treatment efficacy due to acquired resistance.. AC105118.1 is a long non-coding RNA with unknown biological function.. This research attempts to probe into the molecular regulatory mechanism of AC105118.1 in CRC oxaliplatin resistance.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms.
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN-7, -9, and -12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) (Fig. 4).

    Article Title: LncRNA-NEAT1 facilitates autophagy to boost pemetrexed resistance in lung adenocarcinoma via the mir-379-3p/HIF1A pathway.
    Article Snippet: Background: As a primary chemotherapeutic agent for lung adenocarcinoma (LUAD), pemetrexed (PEM) faces the challenge of resistance development in cancer cells due to its chronic use, which compromises its therapeutic benefits.. LncRNA-NEAT1, implicated in the promotion of cancer, is a key player in LUAD.. The objective of this study is to explore the contribution of lncRNA-NEAT1 to PEM resistance in LUAD and to dissect the molecular mechanisms involved.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN - 7, -9, and - 12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) ( ).

    Control:

    Article Title: The assembly and activation of the PANoptosome promote porcine granulosa cell programmed cell death during follicular atresia
    Article Snippet: The coding sequences of the ZBP1 , RIPK3 , and RIPK1 genes, as well as those of their truncation mutants, were amplified from GC cDNA and cloned into the pcDNA3.1 expression vector (Invitrogen).

    Article Title: Generating and characterizing a comprehensive panel of CHO cells glycosylation mutants for advancing glycobiology and biotechnology research
    Article Snippet: The human erythropoietin (EPO) open reading frame (ORF) was cloned into the pcDNA3.1 expression vector (Thermo Fisher Scientific).

    Article Title: Fusobacterium Nucleatum Promotes Microsatellite Instability in Colorectal Carcinoma Through Up-regulation of miRNA-155-5p-Targeted Inhibition of MSH6 via the TLR4/NF-κB Signaling Pathway.
    Article Snippet: Fusobacterium nucleatum (Fn) is significantly associated with poor prognosis in colorectal carcinoma (CRC), however, mechanisms of Fn in DNA mismatch repair (MMR) and microsatellite instability (MSI) in CRC have not been fully elucidated.. Clinical samples are collected to analyze the relationship between Fn abundance and microsatellite stability.. Tumor cells are treated with Fn to detect the expression of proteins related to toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (Myd88), mutS homolog 6 (MSH6), and nuclear factor-κB (NF-κB) signaling pathways, respectively.

    Article Title: Inhibition of myotube formation by platelet-derived growth factor subunit B in QM7 cells
    Article Snippet: Finally, the amplified sequence was inserted into the multicloning site of the pcDNA3.1 expression vector (Invitrogen, Grand Island, NY, USA).

    Article Title: Long non-coding RNA AC105118.1 affects glycolysis to facilitate oxaliplatin resistance in colorectal cancer cells by modulating the miR-378a-3p/KIF26B axis.
    Article Snippet: Background: Oxaliplatin is a first-line chemotherapy drug for colorectal cancer (CRC), but many patients eventually lose treatment efficacy due to acquired resistance.. AC105118.1 is a long non-coding RNA with unknown biological function.. This research attempts to probe into the molecular regulatory mechanism of AC105118.1 in CRC oxaliplatin resistance.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms.
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN-7, -9, and -12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) (Fig. 4).

    Article Title: LncRNA-NEAT1 facilitates autophagy to boost pemetrexed resistance in lung adenocarcinoma via the mir-379-3p/HIF1A pathway.
    Article Snippet: Background: As a primary chemotherapeutic agent for lung adenocarcinoma (LUAD), pemetrexed (PEM) faces the challenge of resistance development in cancer cells due to its chronic use, which compromises its therapeutic benefits.. LncRNA-NEAT1, implicated in the promotion of cancer, is a key player in LUAD.. The objective of this study is to explore the contribution of lncRNA-NEAT1 to PEM resistance in LUAD and to dissect the molecular mechanisms involved.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN - 7, -9, and - 12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) ( ).

    Over Expression:

    Article Title: The assembly and activation of the PANoptosome promote porcine granulosa cell programmed cell death during follicular atresia
    Article Snippet: The coding sequences of the ZBP1 , RIPK3 , and RIPK1 genes, as well as those of their truncation mutants, were amplified from GC cDNA and cloned into the pcDNA3.1 expression vector (Invitrogen).

    Article Title: Generating and characterizing a comprehensive panel of CHO cells glycosylation mutants for advancing glycobiology and biotechnology research
    Article Snippet: The human erythropoietin (EPO) open reading frame (ORF) was cloned into the pcDNA3.1 expression vector (Thermo Fisher Scientific).

    Article Title: Fusobacterium Nucleatum Promotes Microsatellite Instability in Colorectal Carcinoma Through Up-regulation of miRNA-155-5p-Targeted Inhibition of MSH6 via the TLR4/NF-κB Signaling Pathway.
    Article Snippet: Fusobacterium nucleatum (Fn) is significantly associated with poor prognosis in colorectal carcinoma (CRC), however, mechanisms of Fn in DNA mismatch repair (MMR) and microsatellite instability (MSI) in CRC have not been fully elucidated.. Clinical samples are collected to analyze the relationship between Fn abundance and microsatellite stability.. Tumor cells are treated with Fn to detect the expression of proteins related to toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (Myd88), mutS homolog 6 (MSH6), and nuclear factor-κB (NF-κB) signaling pathways, respectively.

    Article Title: Inhibition of myotube formation by platelet-derived growth factor subunit B in QM7 cells
    Article Snippet: Finally, the amplified sequence was inserted into the multicloning site of the pcDNA3.1 expression vector (Invitrogen, Grand Island, NY, USA).

    Article Title: Long non-coding RNA AC105118.1 affects glycolysis to facilitate oxaliplatin resistance in colorectal cancer cells by modulating the miR-378a-3p/KIF26B axis.
    Article Snippet: Background: Oxaliplatin is a first-line chemotherapy drug for colorectal cancer (CRC), but many patients eventually lose treatment efficacy due to acquired resistance.. AC105118.1 is a long non-coding RNA with unknown biological function.. This research attempts to probe into the molecular regulatory mechanism of AC105118.1 in CRC oxaliplatin resistance.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms.
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN-7, -9, and -12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) (Fig. 4).

    Article Title: LncRNA-NEAT1 facilitates autophagy to boost pemetrexed resistance in lung adenocarcinoma via the mir-379-3p/HIF1A pathway.
    Article Snippet: Background: As a primary chemotherapeutic agent for lung adenocarcinoma (LUAD), pemetrexed (PEM) faces the challenge of resistance development in cancer cells due to its chronic use, which compromises its therapeutic benefits.. LncRNA-NEAT1, implicated in the promotion of cancer, is a key player in LUAD.. The objective of this study is to explore the contribution of lncRNA-NEAT1 to PEM resistance in LUAD and to dissect the molecular mechanisms involved.

    Article Title: Identification and characterization of human KALRN mRNA and Kalirin protein isoforms
    Article Snippet: The top-ranked splice isoforms of the most abundant KALRN - 7, -9, and - 12 full-length transcripts in frontal lobe and aorta sample pools were synthesized with an N-terminal DYKDDDDK peptide (FLAG)-tag separated by a 5-glycine spacer by GeneArt (Thermo Fisher Scientific) and cloned into pcDNA3.1 expression vector (Invitrogen) ( ).



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